Brief history of research that challenges the DNA-based circadian clock and the Trancription/translation Feedback Loop model of the clock.
Created by coturnix on Feb 10, 2011
Last updated: 03/18/11 at 01:00 AM
Tags: science circadian genetics chronobiology
O’Neill JS., Gerben van Ooijen, Laura E. Dixon, Carl Troein, Florence Corellou, François-Yves Bouget, Akhilesh B. Reddy and Andrew J. Millar, (2011). Circadian rhythms persist without transcription in a eukaryote, Nature, 469 (554–558), doi:10.1038/nature09654
O’Neill JS, & Reddy AB (2011). Circadian clocks in human red blood cells. Nature, 469 (7331), 498-503 PMID: 21270888
Mori T, Williams DR, Byrne MO, Qin X, Egli M, et al. (2007) Elucidating the Ticking of an In Vitro Circadian Clockwork. PLoS Biol 5(4): e93. doi:10.1371/journal.pbio.0050093
Mehra A, Hong CI, Shi M, Loros JJ, Dunlap JC, et al. (2006) Circadian Rhythmicity by Autocatalysis. PLoS Comput Biol 2(7): e96. doi:10.1371/journal.pcbi.0020096
Revisiting, in fruitflies, the Njus-Sulzman-Hastings (1974) model for membrane-based circadian mechanism, it is clear that the cell membrane is an important component of the model.
Tomita, Jun, Masato Nakajima, Takao Kondo and Hideo Iwasaki (2004) No Transcription-Translation Feedback in Circadian Rhythm of KaiC Phosphorylation, Science. Vol. 307 no. 5707 pp. 251-254, DOI: 10.1126/science.1102540
Knocking out clock genes eliminated the morning bout of activity but not the evening bout of activity in fruitflies. Helfrich-Förster, Charlotte (2001) The locomotor activity rhythm of Drosophila melanogaster is controlled by a dual oscillator system. J Insect Physiol 47:877-887.
Period is a basic property of a circadian rhythm, thought to reside in the clock genes. But inhibition of transcription and translation did not eliminate altered periods ("aftereffects") in the sea slug Bulla gouldiana.
Page, Terry (2000) A Novel Mechanism for the Control of Circadian Clock Period by Light, J Biol Rhythms April 2000 vol. 15 no. 2 155-162, doi: 10.1177/074873040001500209
Kondo, T., Mori, T., Lebedeva, N.V., Aoki, S., Ishiura, M., and Golden, S.S. (1997). Circadian rhythms in rapidly dividing cyanobacteria. Science 275, 224–227.
Mori, T., Binder, B., and Johnson, C.H. (1996) Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours. Proc. Natl. Acad. Sci. USA 93, 10183–10188.
Runft, Linda L., and Dina F. Mandoli (1996) Coordination of cellular events that precede reproductive onset in Acetabularia acetabulum: evidence for a ‘loop’ in development. Development 122, 1187-1194
Woolum JC (1991) A re-examination of the role of the nucleus in generating the circadian rhythm in Acetabularia. J Biol Rhythms 6:129-136.Acta
Peleg, L., A. Dotan, P. Luzato and I. E. Ashkenazi, (1990a) Hexose monophosphate shunt activities in human erythrocytes during oxidative damage induced by hydrogen peroxide, In Vitro Cellular & Developmental Biology - Plant, Volume 26, Number 10, 978-982, DOI: 10.1007/BF02624472
Peleg, L., A. Dotan, P. Luzato and I. E. Ashkenazi (1990b) Long ultradian rhythms in red blood cells and ghost suspensions: Possible involvement of cell membrane , In Vitro Cellular & Developmental Biology - Plant, Volume 26, Number 10, 978-982, DOI: 10.1007/BF02624472
HARTWIG, R., M. SCHWEIGER, R. SCHWEIGER AND H. G. SCHWEIGER (1985) Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia, Proc. Nadl. Acad. Sci. USA, Vol. 82, pp. 6899-6902, October 1985
Ohm-Schradera, L., G. Holzapfela and R. Hardeland (1980) Circadian rhythms in human erythrocytes in vitro not confirmed, Biological Rhythm Research, Volume 11, Issue 3 November 1980 , pages 199 - 207
Mabood SF, Newman PF, Nimmo IA. (1978) Circadian rhythms in the activity of acetylcholinesterase of human erythrocytes incubated in vitro, Biochem Soc Trans. 1978;6(1):305-8.
Daily rhythmic changes in Mg2+-dependent ATPase activity in human red blood cell membranes in vitro
MERGENHAGEN, D. and H. G. SCHWEIGER, (1975a) CIRCADIAN RHYTHM OF OXYGEN EVOLUTION IN CELL FRAGMENTS OF ACETABULARIA MEDITERRANEA, Experimental Cell Research 92:127-130
MERGENHAGEN, D. and H. G. SCHWEIGER, (1975b) THE EFFECT OF DIFFERENT INHIBITORS OF TRANSCRIPTION AND TRANSLATION ON THE EXPRESSION AND CONTROL OF CIRCADIAN RHYTHM IN INDIVIDUAL CELLS OF ACETABULARIA. Experimental Cell Research 94: 321-326
Publication of the Njus-Sulzman-Hastings model of the membrane-based model for the circadian clock.
SWEENEY, B. M. (1974) A physiological model for circadian rhythms from the Acetabularia rhythm paradoxes. Int. J. Chronobiol. 2: 25-33.
VANDEN DRIESSCHE, Therese, and SILVANO BONOTTO, (1969) THE CIRCADIAN RHYTHM IN RNA SYNTHESIS IN ACETABULARIA MEDITERRANEA, Biochim. Biophys. Acta, 179: 58-66
TERBORGH, J., and G. D. McLEOD (1967) The photosynthetic rhythm of Acetabularia crenulata: I. Continuous measurements of oxygen exchange in alternating light-dark regimes and in constant light of different intensities. Biol. Bull. 133: 659-669.
A circadian rhythm of mating type reversals in Paramecium multimicronucleatum, syngen 2, and its genetic control
VANDEN DRIESCHE, T. (1966) The role of the nucleus in the circadian rhythms of Acetabularia mediterranea. Biochim. Biophys. Acta 126: 456-470.
SCHWEIGER, E., H. G. WALRAFF, and H. G. SCHWEIGER (1964b) Endogenous circadian rhythm in cytoplasm of Acetabularia: Influence of the nucleus. Science 61548-665:9.
SWEENEY, B. M., and F. T. HAXO (1961) Persistence of a photosynthetic rhythm in enucleated Acetabularia. Science 134: 1361-1363.
After decades of studying rhythmic phenomena in a variety of organisms, scientists got together at Cold Spring Harbor in 1960 for the foundational meeting of the field. Structure of DNA was new at the time, and comparatively little was known about the inner workings of a cell. But most researchers were eager to break into the 'black box' and start investigating how the circadian clock ticks inside of cells. A number of models were proposed, some focusing on cell membranes, others on DNA. Thus, experiments were started to test the role of nucleus and DNA in the clock mechanism. The other timeline - Discovery of circadian clock genes - shows the successes in the study of clock genetics. This timeline chronicles experimental results that pose difficulties to the DNA-centered view. DNA is important, but not sufficient to explain the circadian clock. Compare and contrast the two timelines.